High-throughput screens for eef-2 kinase

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August undefined, 2024

WebAug 24, 2024 · The High-throughput screening method screens millions of chemical and biological compounds in a short interval of time. It is an automated process and screens many biological or chemical compounds for their therapeutic potential. ... As an example, the high-throughput screening process successfully identified a potent pan-SRC kinase … WebEnter the email address you signed up with and we'll email you a reset link.

High-Throughput Screens for eEF-2 Kinase - SAGE …

WebNov 1, 2016 · Purified human eEF2K was obtained from an expression system and a luminescence-based high-throughput screening (HTS) assay was developed using MH-1 … ion stick

Yeast-based high-throughput screens for discovery of kinase …

WebOct 26, 2015 · High-Throughput Screens for eEF-2 Kinase. Article. Sep 2013; Ramakrishna Edupuganti; Ashwini K Devkota; Mangalika Warthaka; Kevin Dalby; eEF-2 kinase is a potential therapeutic target for breast ... WebTwo high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria. The first is a fluorescence-based assay that uses the phosphorylation of a Sox-based peptide substrate by eEF-2K, which results in a 5-fold increase in fluorescence emission, allowing for continuous monitoring of the kinase activity. WebJun 10, 2014 · High-throughput screening platforms have proved beneficial for protein kinase drug discovery and development, as large compound libraries can be screened with ease and compound potency and selectivity across a subset of the kinome can be determined [13], [14]. on the go mattapoisett

Design and Implementation of High Throughput Screening Assays …

High-throughput screens for eEF-2 kinase - Houston …

WebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput … WebNov 26, 2007 · Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement of kinase activity remains a common high-throughput screening (HTS) application. Recently, enzyme-coupled luciferase-kinase (LK) format assays have been introduced. ion storm black knightWebMay 1, 2014 · eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt Inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. ... High-throughput screens for eEF-2 kinase. J Biomol Screen, 19 (2013), pp. 445-452. Google Scholar. Diggle et al., 1998. on the go mm size

"WebFeb 14, 2024 · To identify kinases that would regulate brown adipocyte differentiation and formation, we developed a high-content and high-throughput in vitro image-based screening system that combined two experimental approaches (fig. S1A). " - High-throughput screens for eef-2 kinase

High-throughput screens for eef-2 kinase

WebJul 13, 2024 · In the same study, the identification of a selective but weak inhibitor of eEF2K (IC 50 = 0.28 μM) called A-484954 through a high-throughput screen was also reported … WebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput …

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WebMay 1, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was … WebSep 1, 2024 · If you are considering a high throughput screen for kinase inhibitors you should really look no further than the PHERAstar. It measures two emission wavelengths at the same time. This is required for the detection modes such as fluorescence polarization (FP) and TR-FRET, which are important in measuring kinases.

WebJan 25, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) is an unusual protein kinase that regulates the elongation stage of protein synthesis by phosphorylating and inhibiting … WebJul 15, 2014 · Eukaryotic elongation factor-2 kinase (eEF2K), encoded by the EEF2K gene, is well-known to be a Ca 2+ /calmodulin (CaM)-dependent kinase which can negatively modulate protein synthesis. It is highly conserved among eukaryotes from mammals to invertebrates, of which human and mouse may have 99 % overall amino acid identity.

WebSep 18, 2024 · A high-throughput screen (kinase reaction performed at room temperature) revealed the compound TCMDS-135051 as a highly selective and specific Pf CLK3 kinase inhibitor. This was confirmed in vivo by the finding that parasite strains that developed resistance against TCMDS-135051 acquired mutations in Pf CLK3. WebHere, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic …

WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of …

WebMay 21, 2016 · Search worldwide, life-sciences literature Search. Advanced Search ions to memorizeWebEvidence that elongation factor 2 kinase (eEF-2K) has potential as a target for anticancer therapy and possibly for the treatment of depression is emerging. Here the steady-state … ions to massWebWe report here the development and optimization of a simple 384-well colorimetric assay to measure H(2)O(2) generated by the redox cycling of compounds incubated with reducing agents in high-throughput screening (HTS) assay buffers. The phenol red-horseradish peroxidase (HRP) assay readily detected H(2)O(2) either added exogenously or generated … ion stick vacuumWebeEF-2 kinase is a highly conserved protein kinase in the calmodulin -mediated signaling pathway that links multiple up-stream signals to the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 ( EEF2) and thus inhibits the EEF2 function. [6] [8] Activation [ edit] ions to goWebAbstract. eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of … on the go moving and storage - redmondWebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of … ions to knowWebHigh-throughput screens for eEF-2 kinase (Q35002039) From Wikidata. Jump to navigation Jump to search. scientific article. edit. Language Label Description Also known as; English: High-throughput screens for eEF-2 kinase. scientific article. Statements. instance of. scholarly article. 1 reference. stated in. on the go monitor mac