Dethiobiotin synthase

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Webdethiobiotin synthetase, dethiobiotin synthase, more top print hide 6 entries Go to Reaction Search REACTION REACTION DIAGRAM COMMENTARY ORGANISM … WebBiotin synthase (BioB) catalyzes the oxidative insertion of a sulfur atom between the C6 methylene and the C9 methyl positions in dethiobiotin. The enzyme couples oxidation …

RCSB PDB - 7JT5: Mycobacterium tuberculosis dethiobiotin …

WebNov 18, 2005 · Working model for the compartmentation of biotin metabolism in the plant cell. Biotin-synthesizing enzymes are as follows: 1, KAPA synthase; 2, DAPA aminotransferase; 3, dethiobiotin synthase; and 4, biotin synthase. The occurrence of reaction 2 in the cytosol and of reaction 3 in mitochondria is predicted from DNA … WebEC Tree. 6 Ligases. 6.3 Forming carbon-nitrogen bonds. 6.3.3 Cyclo-ligases. 6.3.3.3 dethiobiotin synthase. IUBMB Comments. CTP has half the activity of ATP. Specify your search results. Mark a special word or phrase in this record: songs about being betrayed by a friend

RCSB PDB - 1DTS: CRYSTAL STRUCTURE OF AN ATP DEPENDENT …

WebAug 17, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. tuberculosis .Here, we report a binder of Mt DTBS, cyclopentylacetic acid 2 ( K D = 3.4 ± 0.4 mM), identified via in silico screening. X-ray crystallography showed that 2 binds in the … WebMay 1, 2016 · The function of biotin synthase is to convert dethiobiotin, a product of dethiobiotin synthase (encoded by bioD), the third enzyme in the biotin biosynthesis pathway, to biotin. Biotin is an indispensable nutrient found in all living cells. Microorganisms, plants and a few fungi (most fungi obtain it from the environment) are … WebGSE12663: virulence determinants of Francisella tularensis revealed by transcriptional profiling inside macrophages: GSE42863: Flagellar export apparatus protein, FliH, is involved in post-transcriptional regulation of FlaB, motility and virulence of the relapsing fever spirochete Borrelia hermsii smalley automotive point pleasant new jersey

Crystal Structure of Biotin Synthase, an - Science

Biotin, a universal and essential cofactor: synthesis, ligation and

WebMay 1, 2005 · Biotin synthase is an S-adenosyl-L-methionine (SAM) radical enzyme that inserts sulfur into dethiobiotin to produce biotin. The reaction proceeds through 5′-deoxyadenosyl radical intermediates that become reduced during the sulfur insertion step to give another product of the reaction, 5′-deoxyadenosine. We report that Escherichia coli … Biotin synthase (BioB) (EC 2.8.1.6) is an enzyme that catalyzes the conversion of dethiobiotin (DTB) to biotin; this is the final step in the biotin biosynthetic pathway. Biotin, also known as vitamin B7, is a cofactor used in carboxylation, decarboxylation, and transcarboxylation reactions in many organisms including humans. Biotin synthase is an S-Adenosylmethionine (SAM) dependent enzym… smalley avenue alameda countyWebJan 11, 2024 · BioD, dethiobiotin synthase In contrast to the preceding enzymes (with the exception of BioU), BioD (DTB synthase) catalyzes an unusually interesting step. This is the mechanistically unusual reaction of ATP (or CTP) dependent insertion of CO 2 between the N7 and N8 nitrogen atoms of DAPA to form the ureido moiety of biotin. songs about being betrayed by family

"Webd-Desthiobiotin; d-Desthiobiotin is used in affinity chromatography and protein chromatography. d-Desthiobiotin can be used for protein labeling, detection and isolation. " - Dethiobiotin synthase

Dethiobiotin synthase

Biotin synthase: Insights into radical-mediated carbon–sulfur bond ...

WebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin … WebOct 15, 1998 · The crystal structure of the 224-residue protein dethiobiotin synthetase from Escherichia coli has been refined using X-ray diffraction data at 0.97 A resolution at 100 K. The model, consisting of 4143 protein atoms including 1859 H atoms and 436 solvent sites, was refined to a final R factor of 11.6% for all reflections, and has an estimated ...

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WebJan 23, 2007 · Dethiobiotin synthase 1 UniRule annotation. Gene names. Name. bioD1 UniRule annotation. Ordered locus names. b0778, JW0761. Organism names. Organism. Escherichia coli (strain K12) Taxonomic identifier. 83333 NCBI. Taxonomic lineage. Bacteria > Proteobacteria > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > … WebJan 2, 2024 · Keto-aminopelargonic acid synthase, EC 2.3.1.47; diaminopelargonic acid synthase (aminotransferase), EC 2.6.1.62; dethiobiotin synthase, EC 6.3.3.3; and biotin synthase, EC 2.8.1.6. cyclizes by elimination of the phosphate group. The two amide bonds are thus formed using a single mole of ATP ^ ADP + Pj.

WebAug 17, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. …

WebSep 5, 1995 · Dethiobiotin Synthetase: The Carbonylation of 7,8-Diaminononanoic Acid Proceeds Regiospecifically via the N7-Carbamate. Biochemistry 1995, 34 (35) , 10976-10984. In enzymology, a dethiobiotin synthase (EC 6.3.3.3) is an enzyme that catalyzes the chemical reaction ATP + 7,8-diaminononanoate + CO2 ADP + phosphate + dethiobiotin The 3 substrates of this enzyme are ATP, 7,8-diaminononanoate, and CO2, whereas its 3 products are ADP, phosphate, and dethiobiotin.

WebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin synthase is a radical S-adenosylmethionine (SAM) enzyme that reductively cleaves S-adenosylmethionine, generating 5′-deoxyadenosyl radicals that initially abstract a …

WebBiotin synthase, a member of the ‘radical SAM’ (S-adenosylmethionine) family, converts DTB (dethiobiotin) into biotin. The active form of the Escherichia coli enzyme contains two (Fe-S ... smalley avenueWebDethiobiotin synthetase Methods Enzymol. 1979;62:348-52. doi: 10.1016/0076-6879 (79)62241-3. Authors M A Eisenberg , K Krell PMID: 374977 DOI: 10.1016/0076-6879 … smalley avenue liverpoolWebdethiobiotin synthase. Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer. bioD dethiobiotin synthase [] Gene ID: 1245251, discontinued on 29-Dec-2024. Summary. Other designations. dethiobiotin synthase ... smalley bassWebNov 1, 2012 · Biotin synthase is an S-adenosyl-l-methionine (SAM or AdoMet) radical enzyme that catalyzes reduction of the AdoMet sulfonium to produce 5′-deoxyadenosyl radicals, high-energy carbon radicals that can directly … smalley automotive point pleasant njWebJul 1, 2007 · KAPA synthase from A. thaliana was localized in the cytosol by confocal microscopy of the GFP-fused enzyme and by Western blotting (Pinon et al., 2005). Sequence homologs of the bacterial 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin synthase exist in the A. thaliana genome (Pinon et al., 2005). smalley baseball ctWebJan 2, 2004 · The crystal structure of biotin synthase from Escherichia coli in complex with S-adenosyl-L-methionine and dethiobiotin has been determined to 3.4 angstrom resolution.This structure addresses how “AdoMet radical” or “radical SAM” enzymes use Fe 4 S 4 clusters and S-adenosyl-L-methionine to generate organic radicals.Biotin synthase … smalley ave alameda countyWebJun 13, 2005 · Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. Gene Name bioB Uniprot … songs about being blind