Ctfplotter
WebSep 25, 2024 · Herpesviruses present a burden to the general public health as they comprise important human pathogens like herpes simplex virus (HSV-1), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), and cytomegalovirus, the latter being one of the leading viral causes of birth defects in developed countries. WebThe first step to do is to import the tomogram set into the pipeline. To do that, we must first create a description .star file containing the required information, one row per tomogram. The basic fields are the tilt series stack filename, …
Ctfplotter
Did you know?
WebIn addition, analogous to Bayesian polishing and contrast transfer function (CTF) refinement in single-particle analysis, we describe approaches that exploit the increased signal-to-noise ratio in... WebIt is typically the first program to be used when creating a relion tomography data set. It imports an existing alignment from IMOD and the initial CTF parameters from either …
WebSep 9, 2024 · Platelet activation induces reorganization of the cell and the formation of cellular protrusions, pseudopodia. These processes are accompanied by remodeling of the actin cytoskeleton and the activation of platelet integrins, which mediate strong adhesion to the extracellular matrix. WebGo to file Cannot retrieve contributors at this time 21 lines (17 sloc) 852 Bytes Raw Blame This section shows how to import a data set whose tomograms have already been …
WebNOTE: If you want to use ctfplotter for CTF estimation (more robust, but slower than SerialEM's integrated CTFfind) you need to install the 3Dmod standalone version for … WebSep 3, 2024 · Clone via HTTPS Clone with Git or checkout with SVN using the repository’s web address.
WebTomoBEAR is an automated, configurable and customizable full processing pipeline for tomographic cryo-electron microscopy data and subtomogram averaging in the broad field of Cryo-ET based on best practices in the scientific research groups of Misha Kudryashev:
WebMay 19, 2024 · Manual CTF estimation : CTF estimation of a set of input tilt-series using the IMOD procedure. Runs the protocol through the interactive GUI. The resulting defocus values MUST BE SAVED manually by the user. More info: ctfplotter doc Tilt-series preprocess : Normalize input tilt-series and change its storing formatting. More info: … gold 1010WebJul 22, 2024 · Clathrin-mediated endocytosis (CME) is a vital multistep process in all eukaryotes in which plasma membrane cargo proteins are first concentrated into a patch by clathrin adaptors, which are themselves clustered … gold 1011WebRetromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signaling, immunity, neuroprotection, and virus infection. The … hba-consultingWebAligns dose-fractionated data, sorts and stacks aligned frames, determines the defocus of the tilt-series using CTFFIND4, GCTF, or IMOD CTFPLOTTER and then dose-filters the tilt-series in prepartion for alignment using IMOD/eTomo. Options Directories scratch_dir. Absolute path to the folder with the input to be processed. hba.conf in rhelWebApparently relion_tomo_import_tomograms does not read the first line of a ctfplotter output (*.defocus). Adding a first line "#header\n" made it readable. 25 Dec 2024 gold 1016WebJun 2, 2024 · Ctfplotter program from the IMOD suite was used to estimate the defocus of each individual tilt-image in the tilt-series. CTF correction and phase-flipping of the aligned tilt-series was then carried out as part of the IMOD tomographic reconstruction procedure. Though accurate resolution estimation is challenging for tomographic reconstructions ... hba.conf in postgresWebRetromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signaling, immunity, neuroprotection, and virus infection. The retromer core (VPS26/VPS29/VPS35) is present on cargo-transporting, tubular carriers along with a range of sorting nexins. gold 101.3