WebSep 9, 2016 · Here, we describe the heterogeneous effects of CDK4/6 inhibitors, the expression of anti-apoptotic proteins that associate with response to CDK4/6 and MEK inhibitors, and the development of a luciferase-based reporter system to determine the effects of CDK4/6 inhibitors alone and in combination with MEK inhibitors in melanoma … WebMar 14, 2024 · To gain insights into the control of G1 length, we generated an E2F activity reporter that captures free E2F activity after dissociation from Rb sequestration and followed its kinetics of activation at the single-cell level, in real time. Our results demonstrate that its activity is precisely coordinated with S phase progression.
Single-Cell Dynamics Determines Response to CDK4/6 …
WebSep 15, 2024 · A fluorescent CDK2 activity reporter was used for single-cell analysis in conjunction with time-lapse imaging.Results: The luminal androgen receptor (LAR) … WebConsistent with this finding, activation of Cdk4 enhanced the activity of PAX3-FOXO1. In vitro kinase assays revealed that Cdk4 directly phosphorylated PAX3-FOXO1 at Ser(430). brian thorn rit
NF-κB and AKT signaling prevent DNA damage in transformed pre …
WebMar 18, 2024 · A Reporter plasmid-based DNA damage repair assays reveals an increase in NHEJ over HR ... suggesting that p16 down regulation and deregulation of CDK4/6 activity, while accompanying MTX resistance ... WebApr 29, 2011 · Early proliferative events dependent upon CDK4 activity, such as the transition of cells from G0/G1 to S phase, are initiated by de ... dominant negative CDK4 R159N mutant inhibited TLR-induced AP-1 reporter gene activity in a dose dependent manner . Because B cell production ... WebIn these experiments, both p16 and dnCdk4 enhanced the activity of the reporter plasmids. Again, the effect was seen only in quiescent cells, and the enhancement was dependent on the 3′-UTR of the p27 mRNA. We concluded that the inhibition of Cdk4 activity enhances the translation of p27 mRNA through its 3′-UTR in quiescent cells. brian thornson