Bimake mouse direct pcr kit
WebOct 1, 2024 · According to the manufacturer's protocol, DNA was isolated from toes by using a Mouse Direct PCR Kit (Bimake, USA) and amplified by using three sets of primers consisting of the following three sequences: 5′-CTTTGGTAACCATCTGTATGCTGGG-3′ (sense primer for both the wild type and Nrf2 −/− genotypes), 5′ …
Bimake mouse direct pcr kit
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WebOct 20, 2024 · Bimake: 1ml*20 (2000 ractions) EUR 1038: Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. WebJun 22, 2024 · 106 Genomic DNA was obtained by the Mouse Direct PCR Kit (Bimake, B40013, 107 Shanghai) from mouse tail. Ggct-/-mice were genotyped with the following 108 primers: 109 GGCT-KO-F: 5’-TGAGTCATAGATCTGACAGCAAGAG-3’ 110 GGCT-KO-R: 5’-ATAACCCCTGTGAACCATCATTCA-3’ 111 Predicted PCR product size for wild type …
WebJan 18, 2024 · The multiplex PCR was performed in a final reaction volume of 12.5 µL comprising 1× Multiplex PCR Kit buffer (Qiagen, Hilden, ... For a direct comparison of the conventional PCR and the novel ... N.S. Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay. J. Clin ... Web1 hour ago · Genomic DNA from the mouse tail was extracted by using the Mouse Direct PCR Kit (Bimake, TX, USA, #B40015) according to the manufacturer’s instructions. ... #8480) were used as primary antibodies. The following steps used biotin-streptavidin HRP detection kits (ZSGB-BIO, China, SP-9001, SP-9002) according to the manufacturer’s …
WebMouse Direct PCR Kit (For Genotyping) B40015. Bimake. 500 ractions. EUR 490.8. Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the … WebThe Bimake Mouse Direct Pcr Kit reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Direct pcr mouse. Other Bimake products are available in stock.
WebApr 12, 2024 · AAV9-OSM or AAV9-NC was directly injected into the gastrocnemius muscle of each mouse immediately after FAL using a 29-gauge (0.33-mm) needle connected to an insulin syringe. Each mouse was injected with 2.5 × 10 10 vg on each side of the gastrocnemius muscle. Two weeks after injection, the AAV-injected mice were …
WebThe Mouse Direct PCR Kit provides a fast preparation and PCR amplification that is specifically designed for mouse genotyping. The Buffer L and Protease Plus rapidly … fit at fifty menWebMar 31, 2024 · Quantitative Real-Time PCR (qRT-PCR) The total mRNA from NPCs and neuronal cultures was extracted with the RNeasy Plus Mini Kit (ref. 74034, Qiagen). cDNA was generated using the High Capacity cDNA Retro-Transcription Kit, according to the manufacturer’s instructions (Ref. 4368814, ThermoFisher). fit athletic club san diego caWebBimake: 1ml*20 (2000 ractions) EUR 1038: Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. can fidelity manage my 401kWebThe Mouse Direct PCR Kit provides a fast preparation and PCRamplification that is specifically designed for mouse genotyping. TheBuffer L and Protease Plus rapidly … Thank you for Registering with Bimake! close. Products Home > Inhibitor … A: We have performed successful fixation and LacZ staining of embryos spanning … fit at home forchheimWebDescription. The Mouse Direct PCR Kit provides a fast preparation and PCRamplification that is specifically designed for mouse genotyping. TheBuffer L and Protease Plus rapidly digest mouse tail, ear and toe to release intact genomic DNA that can be used directly as the templatefor PCR amplification.By using this kit, the digestion process only ... fit a testWebNov 1, 2024 · Mouse Direct PCR Kit, Bimake, B40015. RIPA lysis buffer, Beyotime, P0013C cOmplete™ EDTA-free Protease Inhibitor Cocktail, Roche, 4693132001 ... Mouse tail tip DNA was extracted using a Mouse Direct PCR Kit, according to the manufacturer's instructions. The genomic region of Gcdh was PCR-amplified (Table S1), ... can fidelity trade hong kong stocksWebMay 27, 2024 · Each individual embryo was transferred to one well of an eight-well PCR strip and lysed using the Mouse Direct PCR Kit (Bimake) according to the manufacturer’s protocols. The target region was ... canfield 100% watersafe lead free solder